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Saito, Yoshimoto*; Hirano, Shinichi*; Nagaoka, Toru*; Amano, Yuki
Ecological Genetics and Genomics, 12, p.100042_1 - 100042_9, 2019/10
Culture-independent molecular techniques enable us to analyze microflora in various environments. Many uncultured prokaryotes have been detected by the molecular methods from extreme environments, including anaerobic, no light, high-pressure, and high temperature. Recently, microbial eukaryotes were also detected in deep-sea environments, suggesting that microbial eukaryotes can adapt to a wider range of environments than previously thought. Here, we performed a culture-independent analysis of eukaryotes from approximately -250 m depth in the Horonobe Underground Research Laboratory at Horonobe, Japan. Our results indicate that fungi are the dominant eukaryotic flora in deep sedimentary rocks of Horonobe. We detected a wide range of species, including Zygomycete, Basidiomycete, and Ascomycete fungi from the rocks. This study is the first report of eukaryotic diversity in deep subsurface sedimentary rocks.
Azad, M. A. K.*; Mazumdar, M. N. N.*; Chaki, A. K.*; Ali, M.*; Hakim, M. L.*; Mamun, A. N. K.*; Hase, Yoshihiro; Nozawa, Shigeki; Tanaka, Atsushi; Koike, Aki*; et al.
SABRAO Journal of Breeding and Genetics, 45(2), p.179 - 186, 2013/06
no abstracts in English
pollen with 
-rays and carbon ionsNaito, Ken*; Kusaba, Makoto*; Shikazono, Naoya; Takano, Toshiya*; Tanaka, Atsushi; Tanisaka, Takatoshi*; Nishimura, Minoru*
Genetics, 169(2), p.881 - 889, 2005/02
Times Cited Count：108 Percentile：88.01(Genetics & Heredity)no abstracts in English
mutants of induced by carbon ionsShikazono, Naoya; Yokota, Yukihiko*; Kitamura, Satoshi; Suzuki, Chihiro*; Watanabe, Hiroshi; Tano, Shigemitsu; Tanaka, Atsushi
Genetics, 163(4), p.1449 - 1455, 2003/04
Irradiation of by carbon ions was carried out to investigate the mutational effect of ion particles in higher plants. Frequencies of embryonic lethals and chlorophyll deficient mutants were found to be significantly higher after carbon ion-irradiation than after electron-irradiation (11-fold and 7.8-fold per unit dose, respectively). During the isolation of mutants, two new classes of flavonoid mutants (
) were isolated from carbon ion-mutagenized M2 plants. From PCR and sequence analysis, two of the three 
mutant alleles were found to have a small deletion within the 
gene and the other was revealed to contain a rearrangement. Using the segregation rates, the mutation rate of carbon ions was estimated to be 17-fold higher than that of electrons. The isolation of novel mutants and the high mutation rate suggest that ion particles can be used as a valuable mutagen for plant genetics.
species revealed by the 5S rDNA spacer sequence and fluorescence in situ hybridizationKitamura, Satoshi; Inoue, Masayoshi*; Shikazono, Naoya; Tanaka, Atsushi
Theoretical and Applied Genetics, 103(5), p.678 - 686, 2001/10
Times Cited Count：46 Percentile：80.85(Agronomy)no abstracts in English
Shikazono, Naoya; Tanaka, Atsushi; Watanabe, Hiroshi; Tano, Shigemitsu
Genetics, 157(1), p.379 - 387, 2001/01
no abstracts in English
